Metabolism Antibodies 1
Anti-AGPAT3 Antibody (CAB14412)
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- SKU:
- CAB14412
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-AGPAT3 Antibody |
| Antibody SKU: | CAB14412 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 145-310 of human AGPAT3 (NP_064517.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, A-549, LO2, U-87MG, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 145-310 of human AGPAT3 (NP_064517.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KRKW EEDR DTVV EGLR RLSD YPEY MWFL LYCE GTRF TETK HRVS MEVA AAKG LPVL KYHL LPRT KGFT TAVK CLRG TVAA VYDV TLNF RGNK NPSL LGIL YGKK YEAD MCVR RFPL EDIP LDEK EAAQ WLHK LYQE KDAL QEIY NQKG MFPG EQFK PARR PWTL LN |
| Gene ID: | 56894 |
| Uniprot: | Q9NRZ7 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus envelope |
| Calculated MW: | 36kDa/43kDa/45kDa |
| Observed MW: | 43kDa |
| Synonyms: | AGPAT3, LPAAT-GAMMA1, LPAAT3 |
| Background: | The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | AGPAT3: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.51; Lipid Metabolism - glycerolipid; Acetyltransferase Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi membrane; membrane; plasma membrane Molecular Function:1-acylglycerol-3-phosphate O-acyltransferase activity Biological Process: phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NRZ7 |
| NCBI GenInfo Identifier: | 12643817 |
| NCBI Gene ID: | 56894 |
| NCBI Accession: | Q9NRZ7.1 |
| UniProt Secondary Accession: | Q9NRZ7,Q3ZCU2, Q6UWP6, Q6ZUC6, Q8N3Q7, Q9NRZ6, D3DSL2 |
| UniProt Related Accession: | Q9NRZ7 |
| Molecular Weight: | 45,514 Da |
| NCBI Full Name: | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma |
| NCBI Synonym Full Names: | 1-acylglycerol-3-phosphate O-acyltransferase 3 |
| NCBI Official Symbol: | AGPAT3 |
| NCBI Official Synonym Symbols: | LPAAT3; LPAAT-GAMMA1 |
| NCBI Protein Information: | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma |
| UniProt Protein Name: | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma |
| UniProt Synonym Protein Names: | 1-acylglycerol-3-phosphate O-acyltransferase 3; 1-AGP acyltransferase 3; 1-AGPAT 3; Lysophosphatidic acid acyltransferase gamma; LPAAT-gamma |
| Protein Family: | 1-acyl-sn-glycerol-3-phosphate acyltransferase |
| UniProt Gene Name: | AGPAT3 |
| UniProt Entry Name: | PLCC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AGPAT3 antibody (CAB14412) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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