Metabolism Antibodies 2

Anti-AGPAT1 Antibody (CAB6517)

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SKU:
CAB6517
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-AGPAT1 Antibody
Antibody SKU:CAB6517
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, SW620, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PIVP IVMS SYQD FYCK KERR FTSG QCQV RVLP PVPT EGLT PDDV PALA DRVR HSML TVFR EIST DGRG GGDY LKKP GGGG
Gene ID:10554
Uniprot:Q99943
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:31kDa
Observed MW:32kDa
Synonyms:AGPAT1, 1-AGPAT1, G15, LPAAT-alpha, LPAATA
Background:This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.
UniProt Protein Function:AGPAT1: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
UniProt Protein Details:

Protein type:Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - glycerolipid; EC 2.3.1.51; Transferase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function:1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: positive regulation of cytokine production; phospholipid metabolic process; glycerophospholipid biosynthetic process; energy reserve metabolic process; positive regulation of cellular metabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; positive regulation of cytokine and chemokine mediated signaling pathway

NCBI Summary:This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:Q99943
NCBI GenInfo Identifier:3914372
NCBI Gene ID:10554
NCBI Accession:Q99943.2
UniProt Secondary Accession:Q99943,Q5BL03, A2BFI5,
UniProt Related Accession:Q99943
Molecular Weight:283
NCBI Full Name:1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
NCBI Synonym Full Names:1-acylglycerol-3-phosphate O-acyltransferase 1
NCBI Official Symbol:AGPAT1  
NCBI Official Synonym Symbols:G15; LPAATA; 1-AGPAT1; LPAAT-alpha  
NCBI Protein Information:1-acyl-sn-glycerol-3-phosphate acyltransferase alpha; 1-AGPAT 1; 1-AGP acyltransferase 1; lysophospholipid acyltransferase; lysophosphatidic acid acyltransferase alpha; 1-acylglycerol-3-phosphate O-acyltransferase 1 (acetoacetly Coenzyme A thiolase); 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
UniProt Protein Name:1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
UniProt Synonym Protein Names:1-acylglycerol-3-phosphate O-acyltransferase 1; 1-AGP acyltransferase 1; 1-AGPAT 1; Lysophosphatidic acid acyltransferase alpha; LPAAT-alpha; Protein G15
Protein Family:1-acyl-sn-glycerol-3-phosphate acyltransferase
UniProt Gene Name:AGPAT1  
UniProt Entry Name:PLCA_HUMAN
Anti-AGPAT1 Antibody (CAB6517)Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (CAB6517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-AGPAT1 Antibody (CAB6517)Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody (CAB6517). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (CAB6517) at a dilition of 1:1000.
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