Epigenetics & Nuclear Signaling Antibodies 2

Anti-AGO1 Antibody (CAB6022)

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SKU:
CAB6022
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-AGO1 Antibody
Antibody SKU:CAB6022
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human AGO1 (NP_036331.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:1000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, K-562, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human AGO1 (NP_036331.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEAG PSGA AAGA YLPP LQQV FQAP RRPG IGTV GKPI KLLA NYFE VDIP KIDV YHYE VDIK PDKC PRRV NREV VEYM VQHF KPQI FGDR KPVY DGKK NIYT VTAL PIGN ERVD FEVT IPGE GKDR IFKV SIKW LAIV SWRM LHEA LVSG QIPV PLES VQAL DVAM RHLA SMRY TPVG RSFF
Gene ID:26523
Uniprot:Q9UL18
Cellular Location:Cytoplasm, P-body
Calculated MW:97kDa
Observed MW:110kDa
Synonyms:AGO1, EIF2C, EIF2C1, GERP95, Q99, hAgo1
Background:This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.
UniProt Code:Q9UL18
NCBI GenInfo Identifier:88984241
NCBI Gene ID:26523
NCBI Accession:Q9UL18.3
UniProt Secondary Accession:Q9UL18,Q5TA57, Q6P4S0,
UniProt Related Accession:Q9UL18
Molecular Weight:857
NCBI Full Name:Protein argonaute-1
NCBI Synonym Full Names:argonaute RISC catalytic component 1
NCBI Official Symbol:AGO1  
NCBI Official Synonym Symbols:Q99; EIF2C; EIF2C1; GERP95  
NCBI Protein Information:protein argonaute-1; hAgo1; eIF2C 1; eIF-2C 1; argonaute1; argonaute 1; putative RNA-binding protein Q99; Golgi Endoplasmic Reticulum protein 95 kDa; eukaryotic translation initiation factor 2C, 1
UniProt Protein Name:Protein argonaute-1
UniProt Synonym Protein Names:Argonaute RISC catalytic component 1; Eukaryotic translation initiation factor 2C 1; eIF-2C 1; eIF2C 1; Putative RNA-binding protein Q99
Protein Family:Protein argonaute
UniProt Gene Name:AGO1  
UniProt Entry Name:AGO1_HUMAN
Anti-AGO1 Antibody (CAB6022)Western blot analysis of extracts of various cell lines, using AGO1 antibody (CAB6022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-AGO1 Antibody (CAB6022)Immunoprecipitation analysis of 600ug extracts of Mouse spleen cells using 3ug AGO1 antibody (CAB6022). Western blot was performed from the immunoprecipitate using AGO1 antibody (CAB6022) at a dilition of 1:500.
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