Developmental Biology
Anti-AGFG1 Antibody (CAB6294)
- SKU:
- CAB6294
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-AGFG1 Antibody |
| Antibody SKU: | CAB6294 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, HepG2, Mouse lung, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS AKRK QEEK HLKM LRDM TGLP HNRK CFDC DQRG PTYV NMTV GSFV CTSC SGSL RGLN PPHR VKSI SMTT FTQQ EIEF LQKH GNEV CKQI WLGL FDDR SSAI PDFR DPQK VKEF LQEK YEKK RWYV PPEQ AKVV ASVH ASIS GSSA SSTS STPE VKPL KSLL GDSA PTLH LNKG TPSQ SPVV GRSQ GQQQ EKKQ FDLL SDLG SDIF AAPA PQST ATAN FANF AHFN SHAA QNSA NADF ANFD AFGQ SSGS SNFG GFPT ASHS PFQP QTTG GSAA SVNA |
| Gene ID: | 3267 |
| Uniprot: | P52594 |
| Cellular Location: | Cytoplasmic vesicle, Nucleus |
| Calculated MW: | 54kDa/58kDa/60kDa |
| Observed MW: | 55kDa |
| Synonyms: | AGFG1, HRB, RAB, RIP |
| Background: | The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | RIP: Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. Interacts with EPS15R and EPS15. Interacts with FCHO1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear export; RNA-binding Chromosomal Location of Human Ortholog: 2q36.3 Cellular Component: intracellular membrane-bound organelle; nuclear pore Molecular Function:protein binding; RNA binding Biological Process: mRNA export from nucleus |
| NCBI Summary: | The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P52594 |
| NCBI GenInfo Identifier: | 26007019 |
| NCBI Gene ID: | 3267 |
| NCBI Accession: | P52594.2 |
| UniProt Secondary Accession: | P52594,Q15277, Q4VAS0, Q4VAS1, Q4VAS3, Q53QT8, Q53R11 B3KUL1, E9PHX7, |
| UniProt Related Accession: | P52594 |
| Molecular Weight: | 60,767 Da |
| NCBI Full Name: | Arf-GAP domain and FG repeat-containing protein 1 |
| NCBI Synonym Full Names: | ArfGAP with FG repeats 1 |
| NCBI Official Symbol: | AGFG1 |
| NCBI Official Synonym Symbols: | HRB; RAB; RIP |
| NCBI Protein Information: | arf-GAP domain and FG repeat-containing protein 1 |
| UniProt Protein Name: | Arf-GAP domain and FG repeat-containing protein 1 |
| UniProt Synonym Protein Names: | HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein |
| Protein Family: | Riparin |
| UniProt Gene Name: | AGFG1 |
 | Western blot analysis of extracts of various cell lines, using AGFG1 antibody (CAB6294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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