Name: Anti-AES Antibody (CAB19944)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19944
Availability: OutOfStock

Antibody Name:	Anti-AES Antibody [KO Validated]
Antibody SKU:	CAB19944
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human AES.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human AES.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	166
Uniprot:	Q08117
Cellular Location:	Nucleus
Calculated MW:	21kDa/29kDa
Observed MW:	22KDa

Anti-AES Antibody [KO Validated] (CAB19944)

Western blot analysis of extracts from normal (control) and AES knockout (KO) HeLa cells, using AES antibody (CAB19944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

AES, AES-1, AES-2, ESP1, GRG, GRG5, Grg-5, TLE5

Background:

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleus Molecular Function:protein binding; transcription corepressor activity Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development
NCBI Summary:	The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q08117
NCBI GenInfo Identifier:	23503062
NCBI Gene ID:	166
NCBI Accession:	Q08117.4
UniProt Secondary Accession:	Q08117,Q12808, Q14CJ1, Q96TG9, Q9UDY9, B2RBL0,
UniProt Related Accession:	Q08117
Molecular Weight:	29,091 Da
NCBI Full Name:	Amino-terminal enhancer of split
NCBI Synonym Full Names:	amino-terminal enhancer of split
NCBI Official Symbol:	AES
NCBI Official Synonym Symbols:	GRG; ESP1; GRG5; TLE5; AES-1; AES-2
NCBI Protein Information:	amino-terminal enhancer of split; gp130-associated protein GAM
UniProt Protein Name:	Amino-terminal enhancer of split
UniProt Synonym Protein Names:	Gp130-associated protein GAM; Protein ESP1; Protein GRG
Protein Family:	Acetyl esterase
UniProt Gene Name:	AES
UniProt Entry Name:	AES_HUMAN