Cell Biology Antibodies 8

Anti-ADRB2 Antibody (CAB2048)

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SKU:
CAB2048
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ADRB2 Antibody
Antibody SKU:CAB2048
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 334-413 of human ADRB2 (NP_000015.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HepG2, Mouse heart, Mouse liver, Rat heart, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 334-413 of human ADRB2 (NP_000015.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IAFQ ELLC LRRS SLKA YGNG YSSN GNTG EQSG YHVE QEKE NKLL CEDL PGTE DFVG HQGT VPSD NIDS QGRN CSTN DSLL
Gene ID:154
Uniprot:P07550
Cellular Location:Cell membrane, Early endosome, Multi-pass membrane protein
Calculated MW:46kDa
Observed MW:53-65kDa
Synonyms:ADRB2, ADRB2R, ADRBR, B2AR, BAR, BETA2AR
Background:This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.
UniProt Protein Function:ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
UniProt Protein Details:

Protein type:Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: lysosome; integral to plasma membrane; apical plasma membrane; plasma membrane; nucleus; receptor complex; endosome

Molecular Function:protein binding; protein homodimerization activity; potassium channel regulator activity; beta2-adrenergic receptor activity; norepinephrine binding; adenylate cyclase binding

Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; transmembrane receptor protein tyrosine kinase activation (dimerization); negative regulation of multicellular organism growth; regulation of vasodilation; adenylate cyclase activation; regulation of sodium ion transport; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of protein ubiquitination; heat generation; negative regulation of smooth muscle contraction; brown fat cell differentiation; endosome to lysosome transport; positive regulation of transcription from RNA polymerase II promoter; response to cold; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure; bone resorption

Disease: Obesity; Asthma, Susceptibility To

NCBI Summary:This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008]
UniProt Code:P07550
NCBI GenInfo Identifier:296439450
NCBI Gene ID:154
NCBI Accession:P07550.3
UniProt Secondary Accession:P07550,O14823, O14824, O14825, O14826, Q4JG18, Q53GA6 Q6GMT4, Q6P4D8, Q8NEQ9, B0LPE4, B2R7X2,
UniProt Related Accession:P07550
Molecular Weight:46,459 Da
NCBI Full Name:Beta-2 adrenergic receptor
NCBI Synonym Full Names:adrenoceptor beta 2, surface
NCBI Official Symbol:ADRB2  
NCBI Official Synonym Symbols:BAR; B2AR; ADRBR; ADRB2R; BETA2AR  
NCBI Protein Information:beta-2 adrenergic receptor; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor; adrenergic, beta-2-, receptor, surface
UniProt Protein Name:Beta-2 adrenergic receptor
UniProt Synonym Protein Names:Beta-2 adrenoreceptor
Protein Family:Beta-2 adrenergic receptor
UniProt Gene Name:ADRB2  
UniProt Entry Name:ADRB2_HUMAN
Anti-ADRB2 Antibody (CAB2048)Western blot analysis of extracts of various cell lines, using ADRB2 antibody (CAB2048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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