Cell Biology Antibodies 4

Anti-ADH5 Antibody (CAB13459)

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SKU:
CAB13459
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ADH5 Antibody
Antibody SKU:CAB13459
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5 (NP_000662.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse brain, Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5 (NP_000662.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANE VIKC KAAV AWEA GKPL SIEE IEVA PPKA HEVR IKII ATAV CHTD AYTL SGAD PEGC FPVI LGHE GAGI VESV GEGV TKLK AGDT VIPL YIPQ CGEC KFCL NPKT NLCQ KIRV TQGK GLMP DGTS RFTC KGKT ILHY MGTS TFSE YTVV ADIS VAKI DPLA PLDK VCLL GCGI STGY GAAV NTAK LEPG SVCA VFGL GGVG LAVI MGCK VAGA SRII GVDI NKDK FARA KEFG ATEC INPQ DFSK PIQE VLIE MTDG GVDY SFEC IGNV KVMR AALE ACHK GWGV SVVV GVAA SGEE IATR PFQL VTGR TWKG TAFG GWKS VESV PKLV SEYM SKKI KVDE FVTH NLSF DEIN KAFE LMHS GKSI RTVV KI
Gene ID:128
Uniprot:P11766
Cellular Location:Cytoplasm
Calculated MW:39kDa
Observed MW:40KDa
Synonyms:ADH5, ADH-3, ADHX, FALDH, FDH, GSH-FDH, GSNOR, HEL-S-60p
Background:This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.
UniProt Protein Function:ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
UniProt Protein Details:

Protein type:Energy Metabolism - methane; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Lipid Metabolism - fatty acid; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.284

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mitochondrion; nucleus

Molecular Function:protein homodimerization activity; electron carrier activity; zinc ion binding; formaldehyde dehydrogenase activity; alcohol dehydrogenase activity; S-(hydroxymethyl)glutathione dehydrogenase activity; fatty acid binding

Biological Process: response to nitrosative stress; respiratory system process; ethanol catabolic process; formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; response to redox state; response to lipopolysaccharide; retinoid metabolic process; ethanol oxidation; positive regulation of blood pressure; aging

NCBI Summary:This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
UniProt Code:P11766
NCBI GenInfo Identifier:113408
NCBI Gene ID:128
NCBI Accession:P11766.4
UniProt Related Accession:P11766
Molecular Weight:
NCBI Full Name:Alcohol dehydrogenase class-3
NCBI Synonym Full Names:alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Symbol:ADH5  
NCBI Official Synonym Symbols:FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p  
NCBI Protein Information:alcohol dehydrogenase class-3
UniProt Protein Name:Alcohol dehydrogenase class-3
UniProt Synonym Protein Names:Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-); FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
UniProt Gene Name:ADH5  
UniProt Entry Name:ADHX_HUMAN
Anti-ADH5 Antibody (CAB13459)Western blot analysis of extracts of various cell lines, using ADH5 antibody (CAB13459) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ADH5 Antibody (CAB13459)Immunohistochemistry of paraffin-embedded rat brain using ADH5 antibody (CAB13459) at dilution of 1:100 (40x lens).
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