Signal Transduction Antibodies 1

Anti-ADAM10 Antibody (CAB10438)

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SKU:
CAB10438
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ADAM10 Antibody
Antibody SKU:CAB10438
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 214-500 of human ADAM10 (NP_001101.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, Raji, Jurkat, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 214-500 of human ADAM10 (NP_001101.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TTSA EKNT CQLY IQTD HLFF KYYG TREA VIAQ ISSH VKAI DTIY QTTD FSGI RNIS FMVK RIRI NTTA DEKD PTNP FRFP NIGV EKFL ELNS EQNH DDYC LAYV FTDR DFDD GVLG LAWV GAPS GSSG GICE KSKL YSDG KKKS LNTG IITV QNYG SHVP PKVS HITF AHEV GHNF GSPH DSGT ECTP GESK NLGQ KENG NYIM YARA TSGD KLNN NKFS LCSI RNIS QVLE KKRN NCFV ESGQ PICG NGMV EQGE ECDC GYSD QCKD ECCF DANQ PEGR KCKL KPG
Gene ID:102
Uniprot:O14672
Cellular Location:Cell membrane, Endomembrane system, Single-pass type I membrane protein
Calculated MW:49kDa/84kDa
Observed MW:70kDa/105kDa
Synonyms:ADAM10, AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz
Background:Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
UniProt Protein Function:ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.
UniProt Protein Details:

Protein type:Cell adhesion; Cell surface; EC 3.4.24.81; Membrane protein, integral; Motility/polarity/chemotaxis; Protease; Vesicle

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: cell surface; cytoplasm; focal adhesion; Golgi apparatus; Golgi-associated vesicle; membrane; nucleus; plasma membrane

Molecular Function:endopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; protein binding; protein homodimerization activity; protein kinase binding

Biological Process: constitutive protein ectodomain proteolysis; ephrin receptor signaling pathway; extracellular matrix disassembly; in utero embryonic development; membrane protein ectodomain proteolysis; monocyte activation; negative regulation of cell adhesion; neutrophil degranulation; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; protein amino acid phosphorylation

Disease: Alzheimer Disease 18; Reticulate Acropigmentation Of Kitamura

NCBI Summary:Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]
UniProt Code:O14672
NCBI GenInfo Identifier:29337031
NCBI Gene ID:102
NCBI Accession:O14672.1
UniProt Secondary Accession:O14672,Q10742, Q92650, B4DU28,
UniProt Related Accession:O14672
Molecular Weight:84kDa
NCBI Full Name:Disintegrin and metalloproteinase domain-containing protein 10
NCBI Synonym Full Names:ADAM metallopeptidase domain 10
NCBI Official Symbol:ADAM10  
NCBI Official Synonym Symbols:RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717  
NCBI Protein Information:disintegrin and metalloproteinase domain-containing protein 10
UniProt Protein Name:Disintegrin and metalloproteinase domain-containing protein 10
UniProt Synonym Protein Names:CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD_antigen: CD156c
Protein Family:Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name:ADAM10  
Anti-ADAM10 Antibody (CAB10438)Western blot analysis of extracts of various cell lines, using ADAM10 antibody (CAB10438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ADAM10 Antibody (CAB10438)Immunofluorescence analysis of HeLa cells using ADAM10 antibody (CAB10438) at dilution of 1:100. Blue: DAPI for nuclear staining.
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