Cell Biology Antibodies 9

Anti-Acetyl-Histone H4-K16 Antibody (CAB5280)

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SKU:
CAB5280
Product Type:
Antibody
Applications:
WB
Applications:
IF
Applications:
IP
Applications:
ChIP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Acetyl-Histone H4-K16 Antibody
Antibody SKU:CAB5280
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP ChIP
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
Application:WB IF IP ChIP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:Hela TSA, HeLa, C2C12 TSA, C2C12
Immunogen:A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GGAK R
Gene ID:8370
Uniprot:P62805
Cellular Location:Chromosome, Nucleus
Calculated MW:11kDa
Observed MW:13kDa
Synonyms:FO108, H4, H4/n, H4F2, H4FN, HIST2H4, Histone H4, HIST1H4A, HIST2H4A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
UniProt Protein Function:Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location: Nucleus. Chromosome.

Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35

Sequence similarities: Belongs to the histone H4 family.

Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]
UniProt Code:P62805
NCBI GenInfo Identifier:77539758
NCBI Gene ID:554313
NCBI Accession:NP_001029249.1
UniProt Secondary Accession:P62805,P02304, P02305, Q6DRA9, Q6FGB8, Q6NWP7, A2VCL0
UniProt Related Accession:P62805
Molecular Weight:11,367 Da
NCBI Full Name:histone H4
NCBI Synonym Full Names:histone cluster 2, H4b
NCBI Official Symbol:HIST2H4B  
NCBI Official Synonym Symbols:H4/o  
NCBI Protein Information:histone H4; histone 2, H4b
UniProt Protein Name:Histone H4
Protein Family:Histone
UniProt Gene Name:HIST1H4A  
UniProt Entry Name:H4_HUMAN
Western blot - Acetyl-Histone H4-K16 Rabbit pAb Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K16 at dilution of 1:100. Blue: DAPI for nuclear staining. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 at dilution of 1:100. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 at dilution of 1:100. Blue: DAPI for nuclear staining. U2OS cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
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