Description
| Antibody Name: | Anti-Acetyl-Histone H2B-K5 Antibody |
| Antibody SKU: | CAB15621 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP ChIP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B |
| Application: | WB IHC IF IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 ChIP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, C6 |
| Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3017/8349 |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 16kDa |
| Synonyms: | |
| Background: |
 | Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. C6 cells were treated by TSA (1 uM) at 37°C for 18 hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |
