Description
Antibody Name: | AF/LE Purified Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody |
Antibody Code: | AGEL1469 |
Size: | 500µg |
Clone: | RB6-8C5 |
Synonyms: | Gr-1,Gr1,Ly-6G/Ly-6C,Ly6G/Ly6C |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | Depletion, FC, WB, IHC, IP |
Concentration: | 1mg/mL |
Isotype: | IgG2a,k |
Conjugation: | Azide Free/Low endotoxin |
Applications: | Depletion, FC, WB, IHC, IP |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | 0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (< 0.002EU/µg) as Determined by LAL gel clotting assay. Purity is > 95% as determined by SDS-PAGE |
Storage: | Store at 2-8°C. Do not freeze. |
Gene ID: | 546644 ,17067 |
Uniprot: | P35461, P0CW02, P0CW03 |
Background: | Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity. |