Description
Antibody Name: | AF/LE Purified Mouse H-2 Monoclonal Antibody |
Antibody Code: | AGEL2108 |
Size: | 500µg |
Clone: | M1/42.3.9.8 |
Synonyms: | Mouse major histocompatibility complex (MHC) H-2, MHC I |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | Block, FC, IF, IP |
Concentration: | 1mg/mL |
Isotype: | IgG2a,k |
Conjugation: | Azide Free/Low endotoxin |
Applications: | Block, FC, IF, IP |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | 0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (< 0.002EU/µg) as Determined by LAL gel clotting assay. Purity is > 95% as determined by SDS-PAGE |
Storage: | Store at 2-8°C. Do not freeze. |
Gene ID: | 111364 |
Background: | The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. |