AF/LE Purified Mouse CD45R/B220 Monoclonal Antibody [RA3.3A 1/6.1](AGEL1400)

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SKU:
AGEL1400
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC

Description

Antibody Name:AF/LE Purified Mouse CD45R/B220 Monoclonal Antibody
Antibody Code:AGEL1400
Size:500µg
Clone:RA3.3A 1/6.1
Synonyms:B220,CD45,GP180,LCA,T200
Host Species:Rat
Species Reactivity:Mouse
Applications:Cell Sep-Neg, Depletion, FC, IHC, IP
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:Azide Free/Low endotoxin
Applications:Cell Sep-Neg, Depletion, FC, IHC, IP
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (< 0.002EU/µg) as Determined by LAL gel clotting assay. Purity is > 95% as determined by SDS-PAGE
Storage:Store at 2-8°C. Do not freeze.
Gene ID:19264,5788
Uniprot:P06800, P08575
Background:CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-2CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.40 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources.
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