AF/LE Purified Anti-Mouse CD162 Antibody [4RA10]

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SKU:
AGEL2164
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Applications:
Block
Reactivity:
Mouse
Host Species:
Rat
Isotype:
Rat IgG1, κ
Clone:
4RA10
Conjugation:
None (Low endotoxin)

Description

Product Name:AF/LE Purified Anti-Mouse CD162 Antibody [4RA10]
Product Code:AGEL2164
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Reactivity:Mouse
Host Species:Rat
Applications:FCM, Block
Isotype:Rat IgG1, κ
Isotype Control:Low Endotoxin Purified Rat IgG1, κ Isotype Control [HRPN] (AGEL2628)
Conjugation:None (Purified antibody-Azide Free/Low endotoxin)
Form:Liquid
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Swissprot:Q62170
Stability & Storage:Keep as concentrated solution. Store at 2-8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Shipping:Biological ice pack at 4°C

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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