Name: Aconitase Assay Kit (BA0070)
Brand: Metabolism Assays
SKU: BA0070
Availability: OutOfStock

Aconitase Assay Kit (BA0070)

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SKU:

BA0070

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Cell Lysate, Tissue Homogenate, Serum

Research Area:

TCA Cycle

Research Area:

Oxidative Stress

Research Area:

ATP and Related

Research Area:

Diabetes & Obesity

Research Area:

Enzyme Activity

Description
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Description

Aconitase Assay Kit - Information

Assay Genie's aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

Applications

For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.

Aconitase Assay Kit - Key Features

Fast and sensitive. Linear detection range (20 sample): 0.5 to 100 U/L for 20 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Aconitase Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL Enzyme A: 120 uL NADP/MTT: 1 mL Enzyme B: 120 uL Standard: 1 mL Substrate: 1 mL
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader.
Method of Detection	OD565nm
Detection Limit	0.5 U/L
Samples	Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Protocol Length	30 min
Size	100 tests
Storage	Store all components at -20°C upon receiving
Shelf Life	6 months

More Details

ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe₄S₄] ²⁺ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich's ataxia and diabetes.

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