Aconitase 1 (ACO1) Monoclonal Antibody
- SKU:
- CAB4821
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IF
Description
| Product Name: | Aconitase 1 (ACO1) Monoclonal Antibody |
| Product Code: | CAB4821 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western blotting, Immunofluorescence |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC2731 |
| Reactivity: | Human, Mouse, Rat |
| Tested Applications: | WB, IF/ICC, ELISA |
| Key Applications: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500-1:1000, IF/ICC 1:50-1:200 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | Mouse liver, Mouse kidney, Rat kidney |
| Cellular Location: | Cytoplasm |
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aconitase 1 (ACO1) (P21399). |
| Sequence: | MSNPF AHLAE PLDPV QPGKK FFNLN KLEDS RYGRL PFSIR VLLEA AIRNC DEFLV KKQDI ENILH WNVTQ HKNIE VPFKP ARVIL QDFTG VPAVV DFAAM |
| Synonyms: | IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1 |
| Calculated MW: | 98kDa |
| Observed MW: | 98kDa |
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