Hormone & Small Molecule ELISA Kits
6-keto-PGF1a/6-keto-prostaglandin F1a ELISA Kit (UNFI0045)
- SKU:
- UNFI0045
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Synonyms:
- 6-keto-PGF1a, 6-keto-prostaglandin F1a
- Reactivity:
- Universal
- Size:
- 48T/96T
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Description
6-keto-PGF1a/6-keto-prostaglandin F1a ELISA Kit
The 6-Keto-PGF1α (6-Keto-Prostaglandin F1α) ELISA Kit from Assay Genie is a reliable tool for accurately measuring levels of 6-Keto-PGF1α in various sample types such as serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it suitable for a wide range of research applications.6-Keto-PGF1α is a metabolite of prostacyclin (PGI2) and serves as a valuable marker for assessing oxidative stress and inflammation, particularly in the context of cardiovascular diseases, thrombotic disorders, and inflammatory conditions.
By measuring 6-Keto-PGF1α levels, researchers can gain insights into the pathophysiology of these diseases and potentially identify new therapeutic targets. In conclusion, the 6-Keto-PGF1α ELISA Kit from Assay Genie is an essential tool for studying the role of prostacyclin metabolism in various pathological conditions, offering accurate and reliable data for advancing biomedical research.
| Product Name: | 6-keto-PGF1a/6-keto-prostaglandin F1a ELISA Kit |
| SKU: | UNFI0045 |
| Reactivity: | Universal |
| Assay Type: | Competitive ELISA, Coated with Antigen |
| Sensitivity: | 9.375 pg/mL |
| Range: | 15.625-1000 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C(Sealed), Don't cryopreserve. |
| Linearity: |
| ||||||||||||||||
| Recovery: |
|
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice. |
| 2 | Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes. |
| 3 | Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash. |
| 4 | Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops. |
| 5 | Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately. |
| 6 | Reading: Measure absorbance at 450 nm using a microplate reader. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately. |
| Plasma | Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Cell Culture Supernatant | Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant. |
| Cell Lysate | Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant. |
| Other Sample Types | Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Lyophilized) | 1 vial | 1 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Purified Water | 200 ul | 200 ul | 2-8°C |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 5 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
Related Products
6-keto-PGF1a (6-keto-prostaglandin F1a) ELISA Kit (UNES00035)
Hormone & Small Molecule ELISA Kits
Human Aldo-keto reductase family 1 member C1 (AKR1C1) ELISA Kit (HUEB1499)
Human Immunology ELISA Kits 7
Human Aldo-keto reductase family 1 member C2 (AKR1C2) ELISA Kit (HUEB2180)
Human Immunology ELISA Kits 9
Human AKR1C4/Aldo-keto reductase family 1 member C4 ELISA Kit (HUFI01881)
Human Aldo-keto reductase family 1 member C3 (AKR1C3) ELISA Kit (HUEB0759)
Human Immunology ELISA Kits 6