Description
| Product Name: | 6*His Monoclonal Antibody |
| Product Code: | MACO0661 |
| Size: | 50µL |
| Target Names: | 6*His |
| Species Reactivity: | N/A |
| Host Species: | Mouse |
| Antigen Species: | N/A |
| Tested Applications: | ELISA, WB; Recommended dilution: WB:1:500-1:2000 |
| Isotype: | IgG2a |
| Clone ID: | 3G5H8 |
| Conjugate: | Non-conjugated |
| Clonality: | Monoclonal |
| Antigen: | HHHHHH(6x His) synthetic peptide conjugate to KLH |
| Form: | Liquid |
| Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | Protein A purified |
| Storage: | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Aliases: | 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag |
| Uniprot ID: | N/A |
| Background: | A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. |
| Research Area: | Others |