The 25-Hydroxy Vitamin D₃ ELISA Kit from Assay Genie is a reliable tool for the quantitative detection of 25-hydroxy vitamin D₃ levels in serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.25-hydroxy vitamin D₃ is a key indicator of vitamin D status in the body, playing a crucial role in regulating calcium and phosphate levels, as well as supporting overall bone health and immune function. Deficiencies in 25-hydroxy vitamin D₃ have been linked to a range of health conditions, making it a valuable biomarker for studying and monitoring vitamin D deficiency-related diseases. With the 25-Hydroxy Vitamin D₃ ELISA Kit, researchers can efficiently measure 25-hydroxy vitamin D₃ levels in samples, enabling them to better understand the impact of vitamin D on health and potentially develop targeted interventions for improving vitamin D status. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit
SKU:
UNES00011
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
