ZRANB1 Antibody (PACO62107)
- SKU:
- PACO62107
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ZRANB1 Antibody (PACO62107) |
| Antibody SKU: | PACO62107 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Ubiquitin thioesterase ZRANB1 protein (111-397AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate. All lanes: ZRANB1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa. Observed band size: 81 kDa. |
 | IHC image of PACO62107 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO62107 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-α signaling. |
| Synonyms: | Ubiquitin thioesterase ZRANB1 (EC 3.4.19.12) (TRAF-binding domain-containing protein) (hTrabid) (Zinc finger Ran-binding domain-containing protein 1), ZRANB1, TRABID |
| UniProt Protein Function: | ZRANB1: Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'- linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling. Belongs to the peptidase C64 family.Protein type: Protease; Ligase; EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 10q26.13Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: protein binding; ubiquitin-specific protease activityBiological Process: cell migration; cytoskeleton organization and biogenesis; positive regulation of Wnt receptor signaling pathway; regulation of cell morphogenesis |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9UGI0 |
| NCBI GenInfo Identifier: | 212276487 |
| NCBI Gene ID: | 54764 |
| NCBI Accession: | Q9UGI0.2 |
| UniProt Secondary Accession: | Q9UGI0,Q5SQP6, Q69YK3, B4DZ98, D3DRF4 |
| UniProt Related Accession: | Q9UGI0 |
| Molecular Weight: | 80,967 Da |
| NCBI Full Name: | Ubiquitin thioesterase ZRANB1 |
| NCBI Synonym Full Names: | zinc finger RANBP2-type containing 1 |
| NCBI Official Symbol: | ZRANB1 |
| NCBI Official Synonym Symbols: | TRABID |
| NCBI Protein Information: | ubiquitin thioesterase ZRANB1 |
| UniProt Protein Name: | Ubiquitin thioesterase ZRANB1 |
| UniProt Synonym Protein Names: | TRAF-binding domain-containing protein; hTrabid; Zinc finger Ran-binding domain-containing protein 1 |
| Protein Family: | Ubiquitin thioesterase |
| UniProt Gene Name: | ZRANB1 |
| UniProt Entry Name: | ZRAN1_HUMAN |
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