ZNF224 Antibody (PACO59936)
- SKU:
- PACO59936
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ZNF224 Antibody (PACO59936) |
| Antibody SKU: | PACO59936 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Zinc finger protein 224 protein (477-607AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59936 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO59936 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells. |
| Synonyms: | Zinc finger protein 224 (Bone marrow zinc finger 2) (BMZF-2) (Zinc finger protein 233) (Zinc finger protein 255) (Zinc finger protein 27) (Zinc finger protein KOX22), ZNF224, BMZF2 KOX22 ZNF233 ZNF255 ZNF27 |
| UniProt Protein Function: | ZNF224: a probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family.Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger proteinChromosomal Location of Human Ortholog: 19q13.2Cellular Component: nuclear membrane; nucleus; transcriptional repressor complexMolecular Function: protein binding; transcription factor activityBiological Process: negative regulation of transcription, DNA-dependent |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q9NZL3 |
| NCBI GenInfo Identifier: | 313104253 |
| NCBI Gene ID: | 7767 |
| NCBI Accession: | Q9NZL3.3 |
| UniProt Secondary Accession: | Q9NZL3,P17033, Q86V10, Q8IZC8, Q9UID9, Q9Y2P6, A6NFW9 |
| UniProt Related Accession: | Q9NZL3 |
| Molecular Weight: | 82,280 Da |
| NCBI Full Name: | Zinc finger protein 224 |
| NCBI Synonym Full Names: | zinc finger protein 224 |
| NCBI Official Symbol: | ZNF224 |
| NCBI Official Synonym Symbols: | BMZF2; KOX22; ZNF27; BMZF-2; ZNF233; ZNF255 |
| NCBI Protein Information: | zinc finger protein 224 |
| UniProt Protein Name: | Zinc finger protein 224 |
| UniProt Synonym Protein Names: | Bone marrow zinc finger 2; BMZF-2; Zinc finger protein 233; Zinc finger protein 255; Zinc finger protein 27; Zinc finger protein KOX22 |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | ZNF224 |
| UniProt Entry Name: | ZN224_HUMAN |
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