Antibody Name:	ZKSCAN3 Antibody (PACO50986)
Antibody SKU:	PACO50986
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human Zinc finger protein with KRAB and SCAN domains 3 protein (8-275AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Mouse heart tissue, Mouse spleen tissue, Mouse lung tissue. All lanes: ZKSCAN3 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 61, 44 kDa. observed band size: 61, 44 kDa..
	Immunofluorescent analysis of Hela cells using PACO50986 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO50986 at dilution of 1:100.

Background:

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migation in various tumors and myelomas.

Synonyms:

Zinc finger protein with KRAB and SCAN domains 3 (Zinc finger and SCAN domain-containing protein 13) (Zinc finger protein 306) (Zinc finger protein 309) (Zinc finger protein 47 homolog) (Zf47) (Zfp-47), ZKSCAN3, ZFP47 ZNF306 ZNF309 ZSCAN13

UniProt Protein Function:	ZNF306: Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression. Belongs to the krueppel C2H2-type zinc-finger protein family.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; nucleus Molecular Function:chromatin binding; DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: lysosome organization and biogenesis; negative regulation of autophagy; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent
UniProt Code:	Q9BRR0
NCBI GenInfo Identifier:	116242859
NCBI Gene ID:	80317
NCBI Accession:	Q9BRR0.2
UniProt Secondary Accession:	Q9BRR0,Q5VXH3, Q92972, Q9H4T3, B2R8W2, B3KVC0, H7BXX1
UniProt Related Accession:	Q9BRR0
Molecular Weight:	43,793 Da
NCBI Full Name:	Zinc finger protein with KRAB and SCAN domains 3
NCBI Synonym Full Names:	zinc finger with KRAB and SCAN domains 3
NCBI Official Symbol:	ZKSCAN3
NCBI Official Synonym Symbols:	ZF47; Zfp47; ZFP306; ZNF306; ZNF309; zfp-47; ZSCAN13; ZSCAN35; dJ874C20.1; dJ874C20.1.
NCBI Protein Information:	zinc finger protein with KRAB and SCAN domains 3
UniProt Protein Name:	Zinc finger protein with KRAB and SCAN domains 3
UniProt Synonym Protein Names:	Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 309; Zinc finger protein 47 homolog; Zf47; Zfp-47
Protein Family:	Zinc finger protein with KRAB and SCAN domains
UniProt Gene Name:	ZKSCAN3
UniProt Entry Name:	ZKSC3_HUMAN