Antibody Name:	XRCC6/XRCC5 Antibody (PACO23829)
Antibody SKU:	PACO23829
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthesized peptide derived from human Ku70/80.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ku70/80 antibody.
	Western blot analysis of extracts from LOVO cells, using Ku70/80 antibody.
	Immunofluorescence analysis of A549 cells, using Ku70/80 antibody.

Background:

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Synonyms:

86 kDa subunit of Ku antigen; ATP-dependent DNA helicase II; 80 kDa subunit; CTC box binding factor 85 kDa subunit; CTC85

UniProt Protein Function:	Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
UniProt Protein Details:	Protein type:Helicase; DNA repair, damage; EC 3.6.4.-; Nuclear receptor co-regulator; DNA-binding; RNA-binding; EC 4.2.99.- Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: cytosol; membrane; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleoplasm; nucleus; transcription factor complex Molecular Function:5'-deoxyribose-5-phosphate lyase activity; damaged DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; protein binding; protein C-terminus binding Biological Process: DNA ligation; DNA recombination; double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein heterotetramerization; regulation of smooth muscle cell proliferation; telomere maintenance
NCBI Summary:	The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
UniProt Code:	P12956
NCBI GenInfo Identifier:	125729
NCBI Gene ID:	2547
NCBI Accession:	P12956.2
UniProt Secondary Accession:	P12956,Q6FG89, Q9UCQ2, Q9UCQ3, B1AHC8,
UniProt Related Accession:	P12956
Molecular Weight:	65,149 Da
NCBI Full Name:	X-ray repair cross-complementing protein 6
NCBI Synonym Full Names:	X-ray repair cross complementing 6
NCBI Official Symbol:	XRCC6
NCBI Official Synonym Symbols:	ML8; KU70; TLAA; CTC75; CTCBF; G22P1
NCBI Protein Information:	X-ray repair cross-complementing protein 6
UniProt Protein Name:	X-ray repair cross-complementing protein 6
UniProt Synonym Protein Names:	5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6
Protein Family:	X-ray repair cross-complementing protein
UniProt Gene Name:	XRCC6
UniProt Entry Name:	XRCC6_HUMAN