WNK4 Antibody (PACO46906)
- SKU:
- PACO46906
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | WNK4 Antibody (PACO46906) |
| Antibody SKU: | PACO46906 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Serine/threonine-protein kinase WNK4 protein (828-1106AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO46906 at dilution of 1:100. |
 | Immunofluorescent analysis of PC-3 cells using PACO46906 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunofluorescent analysis of HepG2 cells using PACO46906 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). |
| Synonyms: | Serine/threonine-protein kinase WNK4 (EC 2.7.11.1) (Protein kinase lysine-deficient 4) (Protein kinase with no lysine 4), WNK4, PRKWNK4 |
| UniProt Protein Function: | WNK4: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Interacts with the C-terminal region of KCNJ1. Interacts with WNK1 and WNK3. Expressed in kidney, colon and skin. Activation requires autophosphorylation of Ser- 335. Phosphorylation of Ser-331 also promotes increased activity. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; Wnk familyChromosomal Location of Human Ortholog: 17q21-q22Cellular Component: tight junction; cytoplasmMolecular Function: protein serine/threonine kinase activity; protein binding; chloride channel inhibitor activity; ATP bindingBiological Process: protein localization; ion homeostasis; regulation of cellular process; ion transport; chloride transport; protein amino acid phosphorylationDisease: Pseudohypoaldosteronism, Type Iib |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q96J92 |
| NCBI GenInfo Identifier: | 34365793 |
| NCBI Gene ID: | 65266 |
| NCBI Accession: | NP_115763.2 |
| UniProt Secondary Accession: | Q96J92,Q8N8X3, Q8N8Z2, Q96DT8, Q9BYS5, B0LPI0 |
| UniProt Related Accession: | Q96J92 |
| Molecular Weight: | 134,739 Da |
| NCBI Full Name: | serine/threonine-protein kinase WNK4 |
| NCBI Synonym Full Names: | WNK lysine deficient protein kinase 4 |
| NCBI Official Symbol: | WNK4 |
| NCBI Official Synonym Symbols: | PHA2B; PRKWNK4 |
| NCBI Protein Information: | serine/threonine-protein kinase WNK4; protein kinase with no lysine 4; protein kinase lysine-deficient 4 |
| UniProt Protein Name: | Serine/threonine-protein kinase WNK4 |
| UniProt Synonym Protein Names: | Protein kinase lysine-deficient 4; Protein kinase with no lysine 4 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | WNK4 |
| UniProt Entry Name: | WNK4_HUMAN |
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