Antibody Name:	WNK3 Antibody (PACO20908)
Antibody SKU:	PACO20908
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:10-1:50
Species Reactivity:	Human, Mouse
Immunogen:	Synthetic peptide of human WNK3

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20908(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 6%SDS-PAGE,Lysate: 40 µg,Lane 1-2: MCF7 cells, 293T cells,Primary antibody: PACO20908(WNK3 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes.
	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20908(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.

Synonyms:

WNK lysine deficient protein kinase 3

UniProt Protein Function:	WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family Chromosomal Location of Human Ortholog: Xp11.22 Cellular Component: adherens junction; cytoplasm; cytosol; tight junction Molecular Function:ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
UniProt Code:	Q9BYP7
NCBI GenInfo Identifier:	353526307
NCBI Gene ID:	65267
NCBI Accession:	Q9BYP7.3
UniProt Secondary Accession:	Q9BYP7,Q5JRC1, Q6JP76, Q8TCX6, Q9HCK6, B1AKG2,
UniProt Related Accession:	Q9BYP7
Molecular Weight:	192,875 Da
NCBI Full Name:	Serine/threonine-protein kinase WNK3
NCBI Synonym Full Names:	WNK lysine deficient protein kinase 3
NCBI Official Symbol:	WNK3
NCBI Official Synonym Symbols:	PRKWNK3
NCBI Protein Information:	serine/threonine-protein kinase WNK3
UniProt Protein Name:	Serine/threonine-protein kinase WNK3
UniProt Synonym Protein Names:	Protein kinase lysine-deficient 3; Protein kinase with no lysine 3
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	WNK3
UniProt Entry Name:	WNK3_HUMAN