Whole Blood Hemoglobin Assay Kit (BA0062)

Whole Blood Hemoglobin Assay Kit - Information

Assay Genie's Whole Blood Hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 570 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Applications

For quantitative determination of hemoglobin (Hb) in whole blood.

Whole Blood Hemoglobin Assay Kit - Key Features

• Non-hazardous. No hazardous chemicals (e.g. potassium cyanide in Drabkin's Reagent) used.
• Simple and high-throughput. The "mix-and-read" procedure involves addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
• Versatility. Assays can be executed in 96-well plate or cuvette.
• High sensitivity. As low as 5 sample is used. Ideal when samples are limited such as mice blood samples.

Whole Blood Hemoglobin Assay Kit - Data Sheet
Kit Includes	HB Reagent: 50 mL Calibrator: 500 mL (12.0 g/dL)
Kit Requires	Pipetting devices and accessories clear flat- bottom 96-well plates (or cuvettes and plate reader or spectrophotometer.
Method of Detection	OD570nm
Detection Limit	0.2g/dL
Samples	Whole blood samples
Species	All
Protocol Length	5 min
Size	250 tests
Storage	Store reagent and calibrator at 4°C.
Shelf Life	12 months

More Details

HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration. Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery.