WDR73 Antibody (PACO63427)
- SKU:
- PACO63427
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | WDR73 Antibody (PACO63427) |
| Antibody SKU: | PACO63427 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human WD repeat-containing protein 73 protein (163-366AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate. All lanes: WDR73 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 kDa. Observed band size: 42 kDa. |
 | Immunofluorescence staining of SH-SY5Y cells with PACO63427 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO63427 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | May play a role in the regulation of microtubule organization and dynamics. |
| Synonyms: | WD repeat-containing protein 73, WDR73 |
| UniProt Protein Function: | WDR73: Belongs to the WD repeat WDR73 family.Chromosomal Location of Human Ortholog: 15q25.2Disease: Galloway-mowat Syndrome |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is thought to contain multiple WD40 repeats. WD40 repeats are motifs that contain 40-60 amino acids, and usually end with Trp-Asp (WD). This protein is found in the cytoplasm during interphase, but accumulates at the spindle poles and astral microtubules during mitosis. Reduced expression of this gene results in abnormalities in the size and morphology of the nucleus. Mutations in this gene have been associated with Galloway-Mowat syndrome PMID: 25466283), which is a rare autosomal recessive disorder that affects both the central nervous system and kidneys. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015] |
| UniProt Code: | Q6P4I2 |
| NCBI GenInfo Identifier: | 74758274 |
| NCBI Gene ID: | 84942 |
| NCBI Accession: | Q6P4I2.1 |
| UniProt Secondary Accession: | Q6P4I2,Q96JZ1, Q9P0B7 |
| UniProt Related Accession: | Q6P4I2 |
| Molecular Weight: | 41,685 Da |
| NCBI Full Name: | WD repeat-containing protein 73 |
| NCBI Synonym Full Names: | WD repeat domain 73 |
| NCBI Official Symbol: | WDR73 |
| NCBI Official Synonym Symbols: | GAMOS; HSPC264 |
| NCBI Protein Information: | WD repeat-containing protein 73 |
| UniProt Protein Name: | WD repeat-containing protein 73 |
| UniProt Synonym Protein Names: | |
| Protein Family: | WD repeat-containing protein |
| UniProt Gene Name: | WDR73 |
| UniProt Entry Name: | WDR73_HUMAN |
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