WDR59 Antibody (PACO63143)
- SKU:
- PACO63143
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | WDR59 Antibody (PACO63143) |
| Antibody SKU: | PACO63143 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human GATOR complex protein WDR59 protein (619-862AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of U251 cells with PACO63143 at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western Blot. Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate. All lanes: WDR59 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110, 65, 63, 47 kDa. Observed band size: 110 kDa. |
| Background: | As a component of the GATOR subcomplex GATOR2, functions within the amino acid, sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid, sensors SESN2 and CASTOR1. |
| Synonyms: | GATOR complex protein WDR59 (WD repeat-containing protein 59), WDR59, KIAA1923 |
| UniProt Protein Function: | WDR59: Belongs to the WD repeat WDR59 family. 4 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16q23.1Molecular Function: protein bindingBiological Process: cellular response to amino acid starvation; positive regulation of TOR signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q6PJI9 |
| NCBI GenInfo Identifier: | 33871400 |
| NCBI Gene ID: | 79726 |
| NCBI Accession: | BC004519 |
| UniProt Secondary Accession: | Q6PJI9,Q71RE7, Q96PW5, Q9BSW6, Q9HA43, B3KRC3 |
| UniProt Related Accession: | Q6PJI9 |
| Molecular Weight: | 46,909 Da |
| NCBI Full Name: | Homo sapiens WD repeat domain 59, mRNA |
| NCBI Synonym Full Names: | WD repeat domain 59 |
| NCBI Official Symbol: | WDR59 |
| NCBI Official Synonym Symbols: | FP977 |
| NCBI Protein Information: | WD repeat-containing protein 59 |
| UniProt Protein Name: | WD repeat-containing protein 59 |
| UniProt Synonym Protein Names: | |
| Protein Family: | WD repeat-containing protein |
| UniProt Gene Name: | WDR59 |
| UniProt Entry Name: | WDR59_HUMAN |
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