vpx Antibody (PACO61842)
- SKU:
- PACO61842
- Product Type:
- Antibody
- Reactivity:
- Virus
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | vpx Antibody (PACO61842) |
| Antibody SKU: | PACO61842 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Human immunodeficiency virus type 2 subtype A |
| Immunogen: | Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx protein (1-112AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: vpx at 2.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa. |
| Background: | Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity). |
| Synonyms: | Protein Vpx (Viral protein X) (X ORF protein), vpx |
| UniProt Protein Function: | Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (). |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P06939 |
| NCBI GenInfo Identifier: | 139451 |
| NCBI Gene ID: | |
| NCBI Accession: | P06939.1 |
| UniProt Secondary Accession: | P06939 |
| UniProt Related Accession: | P06939 |
| Molecular Weight: | 14.8 kDa |
| NCBI Full Name: | Protein Vpx |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Protein Vpx |
| UniProt Synonym Protein Names: | Viral protein X; X ORF protein |
| Protein Family: | Protein |
| UniProt Gene Name: | vpx |
| UniProt Entry Name: | VPX_HV2RO |
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