Name: VPS52 Antibody (PACO58412)
SKU: PACO58412
Availability: OutOfStock

Antibody Name:	VPS52 Antibody (PACO58412)
Antibody SKU:	PACO58412
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Vacuolar protein sorting-associated protein 52 homolog protein (277-377AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO58412 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO58412 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid, hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.

Synonyms:

Vacuolar protein sorting-associated protein 52 homolog (SAC2 suppressor of actin mutations 2-like protein), VPS52, SACM2L

UniProt Protein Function:	VPS52: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS52 family.Chromosomal Location of Human Ortholog: 6p21.3Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; recycling endosome; trans-Golgi network membraneMolecular Function: protein binding; syntaxin bindingBiological Process: endocytic recycling; lysosomal transport
UniProt Protein Details:
NCBI Summary:	This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:	Q8N1B4
NCBI GenInfo Identifier:	74750887
NCBI Gene ID:	6293
NCBI Accession:	Q8N1B4.1
UniProt Secondary Accession:	Q8N1B4,Q53GR4, Q5JPA0, Q5SQW1, Q8IUN6, Q9NPT5, A2BF38 B0UZZ4, B4DNI9
UniProt Related Accession:	Q8N1B4
Molecular Weight:	68,496 Da
NCBI Full Name:	Vacuolar protein sorting-associated protein 52 homolog
NCBI Synonym Full Names:	VPS52, GARP complex subunit
NCBI Official Symbol:	VPS52
NCBI Official Synonym Symbols:	ARE1; SAC2; SACM2L; dJ1033B10.5
NCBI Protein Information:	vacuolar protein sorting-associated protein 52 homolog
UniProt Protein Name:	Vacuolar protein sorting-associated protein 52 homolog
UniProt Synonym Protein Names:	SAC2 suppressor of actin mutations 2-like protein
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS52
UniProt Entry Name:	VPS52_HUMAN