VPS33A Antibody (PACO50890)
- SKU:
- PACO50890
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | VPS33A Antibody (PACO50890) |
| Antibody SKU: | PACO50890 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Vacuolar protein sorting-associated protein 33A protein (1-300AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human liver cancer using PACO50890 at dilution of 1:100. |
 | Immunofluorescent analysis of U251 cells using PACO50890 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG. |
| Synonyms: | Vacuolar protein sorting-associated protein 33A (hVPS33A), VPS33A |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017] |
| UniProt Code: | Q96AX1 |
| NCBI GenInfo Identifier: | 18105056 |
| NCBI Gene ID: | 65082 |
| NCBI Accession: | NP_075067 |
| UniProt Secondary Accession: | Q96AX1 |
| UniProt Related Accession: | Q96AX1 |
| Molecular Weight: | Observed: 68 kDaPredicted: 68 kDa |
| NCBI Full Name: | vacuolar protein sorting-associated protein 33A isoform 1 |
| NCBI Synonym Full Names: | VPS33A core subunit of CORVET and HOPS complexes |
| NCBI Official Symbol: | VPS33A |
| NCBI Official Synonym Symbols: | MPSPS |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 33A |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 33A |
| UniProt Synonym Protein Names: | |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS33A |
| UniProt Entry Name: | VP33A_HUMAN |
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