Antibody Name:	VAMP2 Antibody (PACO20849)
Antibody SKU:	PACO20849
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthetic peptide of human VAMP2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20849(VAMP2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 12%SDS-PAGE,Lysate: 40 µg,Lane 1-3: Human fetal brain tissue, Mouse brain tissue, Hela cells,Primary antibody: PACO20849(VAMP2 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 30 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO20849(VAMP2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.

Synonyms:

vesicle-associated membrane protein 2 (synaptobrevin 2)

UniProt Protein Function:	VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: zymogen granule membrane; neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to plasma membrane; secretory granule membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction Molecular Function:calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle fusion; regulation of exocytosis; vesicle-mediated transport; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion
NCBI Summary:	The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]
UniProt Code:	P63027
NCBI GenInfo Identifier:	288558837
NCBI Gene ID:	6844
NCBI Accession:	P63027.3
UniProt Secondary Accession:	P63027,P19065, Q9BUC2,
UniProt Related Accession:	P63027
Molecular Weight:	13kDa
NCBI Full Name:	Vesicle-associated membrane protein 2
NCBI Synonym Full Names:	vesicle-associated membrane protein 2 (synaptobrevin 2)
NCBI Official Symbol:	VAMP2
NCBI Official Synonym Symbols:	SYB2; VAMP-2
NCBI Protein Information:	vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; vesicle associated membrane protein 2
UniProt Protein Name:	Vesicle-associated membrane protein 2
UniProt Synonym Protein Names:	Synaptobrevin-2
Protein Family:	Vesicle-associated membrane protein
UniProt Gene Name:	VAMP2
UniProt Entry Name:	VAMP2_HUMAN