Antibody Name:	USP45 Antibody (PACO17402)
Antibody SKU:	PACO17402
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:	Human, Mouse
Immunogen:	Full length fusion protein

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using PACO17402(USP45 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO17402(USP45 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).

Background:

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. A wide range of enzymes facilitate the proteolytic Ub pathway, including USPs (ubiquitin specific peptidases), which are cysteine proteases that are responsible for the release of ubiquitin from a ubiquitylated substrate and precursor fusion proteins. USP45 (Ubiquitin carboxyl-terminal hydrolase 45), also known as Deubiquitinating enzyme 45, is a 819 amino acid, protein that is involved in ubiquitin-dependent protein catabolism. USP45 differs from other USPs in that it contains a UBP-type zinc finger, a domain which binds ubiquitin. Although USP45 is broadly expressed, the highest levels can be found in skeletal muscle, spleen and ovary. There are three isoforms of USP45 which are produced as a result of alternative splicing.

Synonyms:

ubiquitin specific peptidase 45

UniProt Protein Function:	USP45: Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.19.12; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6q16.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ubiquitin-specific protease activity Biological Process: DNA repair; protein deubiquitination
UniProt Code:	Q70EL2
NCBI GenInfo Identifier:	296453002
NCBI Gene ID:	85015
NCBI Accession:	Q70EL2.3
UniProt Secondary Accession:	Q70EL2,Q5T062, Q86T44, Q86TC0, Q9BRU1, B2RXG0,
UniProt Related Accession:	Q70EL2
Molecular Weight:	55,812 Da
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 45
NCBI Synonym Full Names:	ubiquitin specific peptidase 45
NCBI Official Symbol:	USP45
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 45
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 45
UniProt Synonym Protein Names:	Deubiquitinating enzyme 45; Ubiquitin thioesterase 45; Ubiquitin-specific-processing protease 45
Protein Family:	Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:	USP45
UniProt Entry Name:	UBP45_HUMAN