Enzymes Recombinant Proteins

Urokinase Recombinant Protein (RPPB2415)

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RPPB2415

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Product Name:Urokinase Recombinant Protein
Product Code:RPPB2415
Size:5µg
Target:Urokinase
Synonyms:PLAU, ATF, BDPLT5, QPD, u-PA, UPA, URK, Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A, Urokinase-type plasminogen activator chain B.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:Urokinase protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA LLEHHHHHH

Urokinase (UK) is a serine protease, which is one of biological plasminogen activators.It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing.It can be obtained from human urine or kidney cell culture.

Urokinase Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 419 amino acids (21-431) and having a molecular mass of 47.4kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).Urokinase is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.�

UniProt Protein Function:Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
UniProt Protein Details:

Catalytic activity: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation: Inhibited by SERPINA5. Ref.17 Ref.22

Subunit structure: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR. Ref.20 Ref.21

Subcellular location: Secreted.

Tissue specificity: Expressed in the prostate gland and prostate cancers. Ref.23

Post-translational modification: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Involvement in Disease: Quebec platelet disorder (QPD) [MIM:601709]: An autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.24

Pharmaceutical use: Available under the name Abbokinase (Abbott). Used in Pulmonary Embolism (PE) to initiate fibrinolysis. Clinically used for therapy of thrombolytic disorders.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 EGF-like domain.Contains 1 kringle domain.Contains 1 peptidase S1 domain.

NCBI Summary:This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:P00749
NCBI GenInfo Identifier:254763341
NCBI Gene ID:5328
NCBI Accession:P00749.2
UniProt Secondary Accession:P00749,Q15844, Q16618, Q53XS3, Q5SWW9, Q969W6, B4DPZ2
UniProt Related Accession:P00749
Molecular Weight:48,507 Da
NCBI Full Name:Urokinase-type plasminogen activator
NCBI Synonym Full Names:plasminogen activator, urokinase
NCBI Official Symbol:PLAU��
NCBI Official Synonym Symbols:ATF; QPD; UPA; URK; u-PA; BDPLT5��
NCBI Protein Information:urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
UniProt Protein Name:Urokinase-type plasminogen activator
Protein Family:Urokinase-type plasminogen activator
UniProt Gene Name:PLAU��
UniProt Entry Name:UROK_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Uniprot:
P00749
Research Area:
Enzymes
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