Uric Acid Assay Kit - Fluorometric (MAES0005)

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SKU:
MAES0005
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine
Sensitivity:
0.03 umol/L
Range:
0.03-15 umol/L
Assay Time:
40 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Research Area:
ATP and Related
Research Area:
Diabetes & Obesity
Research Area:
Clinical Chemistry

Description

system_update_altDatasheet
Product Name:Uric Acid Assay Kit - Fluorometric
Product Code:MAES0005
Product Size:96 Assays
Sample Type:Serum, plasma, urine, animal tissue
Assay Time:40 min
Instrument:Fluorescence Microplate Reader
Sensitivity:0.03 µmol/L
Detection Range:0.03-15 µmol/L
Recovery Rate:101
Inter CV:7.2
Inter CV:1.5

Uric acid, a purine metabolite, is degraded into allantoin by uricase in most mammals. Due to the absence of urate oxidase gene, uric acid is the final product of purine metabolism in humans, so the level of uric acid in human blood is higher than that in most mammals. Uric acid is a physiologically important plasma antioxidant that effectively protects biological targets from the oxidation of hydroxyl radicals, hypochloric acid and peroxynitrite.

Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Kidney Biomarkers

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