Antibody Name:	URI1 Antibody (PACO55866)
Antibody SKU:	PACO55866
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Unconventional prefoldin RPB5 interactor 1 protein (180-298AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: URI1 antibody at 5.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 52, 57, 54 kDa. Observed band size: 52 kDa.
	IHC image of PACO55866 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO55866 diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.

Synonyms:

Unconventional prefoldin RPB5 interactor 1 (Protein NNX3) (Protein phosphatase 1 regulatory subunit 19) (RNA polymerase II subunit 5-mediating protein) (RPB5-mediating protein), URI1, C19orf2 NNX3 PPP1R19 RMP URI

UniProt Protein Function:	RMP: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occurs in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT. Ubiquitous. Expressed in ovarian cancers. Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells. Belongs to the RNA polymerase II subunit 5-mediating protein family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19q12 Cellular Component: mitochondrion; dendrite; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:phosphatase inhibitor activity; protein binding; chromatin binding; protein phosphatase inhibitor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; response to virus; regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]
UniProt Code:	O94763
NCBI GenInfo Identifier:	239938855
NCBI Gene ID:	8725
NCBI Accession:	O94763.3
UniProt Secondary Accession:	O94763,Q8TC23, Q9UNU3, A8K805, H7BY42,
UniProt Related Accession:	O94763
Molecular Weight:	535
NCBI Full Name:	Unconventional prefoldin RPB5 interactor 1
NCBI Synonym Full Names:	URI1, prefoldin-like chaperone
NCBI Official Symbol:	URI1
NCBI Official Synonym Symbols:	RMP; URI; NNX3; C19orf2; PPP1R19
NCBI Protein Information:	unconventional prefoldin RPB5 interactor 1; RPB5-mediating protein; protein phosphatase 1 regulatory subunit 19; protein phosphatase 1, regulatory subunit 19; RNA polymerase II subunit 5-mediating protein
UniProt Protein Name:	Unconventional prefoldin RPB5 interactor 1
UniProt Synonym Protein Names:	Protein NNX3; Protein phosphatase 1 regulatory subunit 19; RNA polymerase II subunit 5-mediating protein; RPB5-mediating protein
Protein Family:	Unconventional prefoldin RPB5 interactor
UniProt Gene Name:	URI1
UniProt Entry Name:	RMP_HUMAN