Urease Activity Assay Kit (BA0061)

Urease Activity Assay - Information

Assay Genie's Urease Activity Assay Kit provides a very sensitive and convenient means to measure urease activity in a variety of samples including soil. In the assay, urease reacts with urea, resulting in the formation of ammonia, which is determined by the Berthelot method at 670nm. The assay is simple, sensitive, stable and high-throughput adaptable

Applications

For quantitative determination of urease activity and evaluation/screen for urease inhibitors.

Urease Activity Assay - Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified.
• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Urease Activity Assay - Data Sheet
Kit Includes	Assay Buffer: 20 mL (pH 7.0) Reagent A: 12 mL Urea: 1.5 mL Reagent B: 6 mL NH4Cl: 100 mL 50 mM
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plate.
Method of Detection	OD670nm
Detection Limit	0.003 U/L
Samples	Biological, environment etc
Species	All
Protocol Length	40 min
Size	100 tests
Storage	Store all reagent at 4°C
Shelf Life	6 months

More Details

UREASE (Amidohydrolase, EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.(NH₂)₂)CO + H₂)O CO₂) + 2NH₃). Many gastrointestinal or urinary tract pathogens produce urease. Thus its activity is a useful diagnostic parameter for the presence of pathogens such as Helicobacter pylori. Urease is found in bacteria, yeast, and higher plants. Urease activity is commonly determined in anaerobes of the bovine rumen, human feces and environmental samples such as soils and phytoplanktons.