Antibody Name:	UBASH3A Antibody (PACO55042)
Antibody SKU:	PACO55042
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Ubiquitin-associated and SH3 domain-containing protein A protein (287-455AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunofluorescent analysis of HepG2 cells using PACO55042 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Western Blot. Positive WB detected in: K562 whole cell lysate, HL60 whole cell lysate, Hela whole cell lysate, Rat heart tissue. All lanes: UBASH3A antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 75, 70, 60 kDa. Observed band size: 70 kDa.

Background:

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.

Synonyms:

Ubiquitin-associated and SH3 domain-containing protein A (Cbl-interacting protein 4) (CLIP4) (Suppressor of T-cell receptor signaling 2) (STS-2) (T-cell ubiquitin ligand 1) (TULA-1), UBASH3A, STS2

UniProt Protein Function:	STS2: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein phosphatase, tyrosine (non-receptor)Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: cytosolMolecular Function: protein binding
UniProt Protein Details:
NCBI Summary:	This gene encodes one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. Both family members can negatively regulate T-cell signaling. This family member can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:	P57075
NCBI GenInfo Identifier:	10720330
NCBI Gene ID:	53347
NCBI Accession:	P57075.1
UniProt Secondary Accession:	P57075,Q6HA34, Q6HA35, Q6ISI6, Q6ISK3, Q6ISS9, G5E9E4
UniProt Related Accession:	P57075
Molecular Weight:	59,167 Da
NCBI Full Name:	Ubiquitin-associated and SH3 domain-containing protein A
NCBI Synonym Full Names:	ubiquitin associated and SH3 domain containing A
NCBI Official Symbol:	UBASH3A
NCBI Official Synonym Symbols:	TULA; CLIP4; STS-2; TULA-1
NCBI Protein Information:	ubiquitin-associated and SH3 domain-containing protein A
UniProt Protein Name:	Ubiquitin-associated and SH3 domain-containing protein A
UniProt Synonym Protein Names:	Cbl-interacting protein 4; CLIP4; Suppressor of T-cell receptor signaling 2; STS-2; T-cell ubiquitin ligand 1; TULA-1
Protein Family:	Ubiquitin-associated and SH3 domain-containing protein
UniProt Gene Name:	UBASH3A
UniProt Entry Name:	UBS3A_HUMAN