TYW5 Antibody (PACO59684)
- SKU:
- PACO59684
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | TYW5 Antibody (PACO59684) |
| Antibody SKU: | PACO59684 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human tRNA wybutosine-synthesizing protein 5 protein (1-152AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of Hela cells with PACO59684 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. |
| Synonyms: | tRNA wybutosine-synthesizing protein 5 (hTYW5) (EC 1.14.11.42) (tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase), TYW5, C2orf60 |
| UniProt Protein Function: | TYW5: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. Belongs to the TYW5 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 1.14.11.42Chromosomal Location of Human Ortholog: 2q33.1Molecular Function: protein homodimerization activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA bindingBiological Process: wybutosine biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | A2RUC4 |
| NCBI GenInfo Identifier: | 89242134 |
| NCBI Gene ID: | 129450 |
| NCBI Accession: | NP_001034782 |
| UniProt Secondary Accession: | A2RUC4 |
| UniProt Related Accession: | A2RUC4 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | tRNA wybutosine-synthesizing protein 5 |
| NCBI Synonym Full Names: | tRNA-yW synthesizing protein 5 |
| NCBI Official Symbol: | TYW5 |
| NCBI Official Synonym Symbols: | hTYW5; C2orf60 |
| NCBI Protein Information: | tRNA wybutosine-synthesizing protein 5 |
| UniProt Protein Name: | tRNA wybutosine-synthesizing protein 5 |
| UniProt Synonym Protein Names: | tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase |
| Protein Family: | tRNA wybutosine-synthesizing protein |
| UniProt Gene Name: | TYW5 |
| UniProt Entry Name: | TYW5_HUMAN |
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