TUT1 Antibody (PACO23491)
- SKU:
- PACO23491
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | TUT1 Antibody (PACO23491) |
| Antibody SKU: | PACO23491 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from internal of human TUT1. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy. |
 | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody. |
| Background: | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation. |
| Synonyms: | U6 snRNA-specific terminal uridylyltransferase 1; EC 2.7.7.52; U6-TUTase; RNA-binding protein 21; RNA-binding motif protein 21 |
| UniProt Protein Function: | RBM21: Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Does not play a role in replication-dependent histone mRNA degradation.Protein type: Nucleolus; EC 2.7.7.52; EC 2.7.7.19; TransferaseChromosomal Location of Human Ortholog: 11q12.2Cellular Component: nucleoplasm; intercellular bridge; nucleolus; nuclear speck; mRNA cleavage and polyadenylation specificity factor complexMolecular Function: polynucleotide adenylyltransferase activity; protein binding; enzyme binding; mRNA 3'-UTR binding; RNA binding; metal ion binding; RNA uridylyltransferase activity; ATP bindingBiological Process: snRNA processing; mRNA polyadenylation; mRNA cleavage |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009] |
| UniProt Code: | Q9H6E5 |
| NCBI GenInfo Identifier: | 226371750 |
| NCBI Gene ID: | 64852 |
| NCBI Accession: | NP_073741.2 |
| UniProt Secondary Accession: | Q9H6E5,Q2NL65, Q7L583, Q9H6H7, A1A527, A8K995 |
| UniProt Related Accession: | Q9H6E5 |
| Molecular Weight: | 95kd |
| NCBI Full Name: | speckle targeted PIP5K1A-regulated poly(A) polymerase |
| NCBI Synonym Full Names: | terminal uridylyl transferase 1, U6 snRNA-specific |
| NCBI Official Symbol: | TUT1 |
| NCBI Official Synonym Symbols: | PAPD2; RBM21; URLC6; TUTase; STARPAP |
| NCBI Protein Information: | speckle targeted PIP5K1A-regulated poly(A) polymerase |
| UniProt Protein Name: | Speckle targeted PIP5K1A-regulated poly(A) polymerase |
| UniProt Synonym Protein Names: | RNA-binding motif protein 21; RNA-binding protein 21; U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52); U6-TUTase |
| Protein Family: | Speckle targeted PIP5K1A-regulated poly(A) polymerase |
| UniProt Gene Name: | TUT1 |
| UniProt Entry Name: | STPAP_HUMAN |
| Antibodies |
| TUT1 Antibody (PACO05061) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
SIM1 Antibody
Overview Product Name:SIM1 AntibodyProduct Code:PACO64949Size:50µLTarget Names:SIM1Species Reactivity:HumanHost
eutC Antibody
Overview Product Name:eutC AntibodyProduct Code:PACO64709Size:50µLTarget Names:eutCSpecies Reactivity:Escherichia coli
EPHA10 Antibody
Overview Product Name:EPHA10 AntibodyProduct Code:PACO64693Size:50µLTarget Names:EPHA10Species Reactivity:HumanH
otsB Antibody
Overview Product Name:otsB AntibodyProduct Code:PACO64885Size:50µLTarget Names:otsBSpecies Reactivity:Escherichia coli