TUNEL 1-Step In Situ Apoptosis Kit (FL488)

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SKU:
AKES072
Product Type:
Assay
Detection Method:
Fluorometric
Sample Type:
Paraffin Section, Frozen Section, Cell Slide
Research Area:
Apoptosis
Research Area:
TUNEL 1-Step Kits

Description

system_update_altDatasheet
Product Name:TUNEL 1-Step In Situ Apoptosis Kit (FL488)
Product Code:AKES072
Size:20T, 50T, 100T
Application:DNA Fragmentation
Detection Method: Fluorometric Method
Sample Type:Paraffin section, frozen section, cell slide
Assay Time: 3 hours
Detection Instrument:Fluorescence Microscope
Dye Type:FL488
Ex/EM (nm):495/519
Channel Set: FITC
Filter Set: FITC
Other Reagents Required:4% Paraformaldehyde, 0.2% Triton X-100, PBS, ddH2O, Mounting solution with anti-fluorescence quencher

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

Storage:RT/-20°C. Refer to the Manual.
Shipping:Ice pack
Expiration:12 months
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained. Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue). This photo was taken by confocal microscope. Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue). This photo was taken by confocal microscope.
Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue). Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).
Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells. Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.
Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue). Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).
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