TTF1 Transcription Factor Activity Assay
- SKU:
- TFAB00191
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- TTF1
- Reactivity:
- Human
Description
| Product Name: | TTF1 Transcription Factor Activity Assay |
| Product Code: | TFAB00191 |
| Target: | TTF1 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie TTF1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | TTF1: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. |
| UniProt Protein Details: | Protein type:Nucleolus; DNA-binding Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane Molecular Function:chromatin binding; DNA binding Biological Process: cellular protein metabolic process; chromatin remodeling; gene expression; negative regulation of DNA replication; negative regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription initiation from RNA polymerase I promoter; transcription termination |
| NCBI Summary: | This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011] |
| UniProt Code: | Q15361 |
| NCBI GenInfo Identifier: | 328927005 |
| NCBI Gene ID: | 7270 |
| NCBI Accession: | NP_001192225.1 |
| UniProt Secondary Accession: | Q15361,Q4VXF3, Q58EY2, Q6P5T5, A1L160, |
| UniProt Related Accession: | Q15361 |
| Molecular Weight: | 103,051 Da |
| NCBI Full Name: | transcription termination factor 1 isoform 2 |
| NCBI Synonym Full Names: | transcription termination factor, RNA polymerase I |
| NCBI Official Symbol: | TTF1 |
| NCBI Official Synonym Symbols: | TTF-1; TTF-I |
| NCBI Protein Information: | transcription termination factor 1 |
| UniProt Protein Name: | Transcription termination factor 1 |
| UniProt Synonym Protein Names: | RNA polymerase I termination factor; Transcription termination factor I; TTF-I |
| Protein Family: | Transcription termination factor |
| UniProt Gene Name: | TTF1 |
| UniProt Entry Name: | TTF1_HUMAN |
