Name: TSEN15 Antibody (PACO59289)
SKU: PACO59289
Availability: OutOfStock

Antibody Name:	TSEN15 Antibody (PACO59289)
Antibody SKU:	PACO59289
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human tRNA-splicing endonuclease subunit Sen15 protein (1-171AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO59289 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO59289 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Synonyms:

tRNA-splicing endonuclease subunit Sen15 (SEN15 homolog) (HsSEN15) (tRNA-intron endonuclease Sen15), TSEN15, C1orf19 SEN15

UniProt Protein Function:	Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini (PubMed:15109492, PubMed:27392077). There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (PubMed:15109492).
UniProt Protein Details:
NCBI Summary:	This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17. [provided by RefSeq, Jul 2014]
UniProt Code:	Q8WW01
NCBI GenInfo Identifier:	24308390
NCBI Gene ID:	116461
NCBI Accession:	NP_443197
UniProt Secondary Accession:	Q8WW01,Q9BZQ5, B4DKP0
UniProt Related Accession:	Q8WW01
Molecular Weight:	20.9kDa (191aa), confirmed by MALDI-TOF
NCBI Full Name:	tRNA-splicing endonuclease subunit Sen15 isoform 1
NCBI Synonym Full Names:	tRNA splicing endonuclease subunit 15
NCBI Official Symbol:	TSEN15
NCBI Official Synonym Symbols:	PCH2F; sen15; C1orf19
NCBI Protein Information:	tRNA-splicing endonuclease subunit Sen15
UniProt Protein Name:	tRNA-splicing endonuclease subunit Sen15
UniProt Synonym Protein Names:	SEN15 homolog; HsSEN15; tRNA-intron endonuclease Sen15
Protein Family:	tRNA-splicing endonuclease
UniProt Gene Name:	TSEN15
UniProt Entry Name: