TRIM8 Antibody (PACO57480)
- SKU:
- PACO57480
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | TRIM8 Antibody (PACO57480) |
| Antibody SKU: | PACO57480 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 protein (367-517AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of HepG2 cells with PACO57480 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57480 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. |
| Synonyms: | E3 ubiquitin-protein ligase TRIM8 (EC 2.3.2.27) (Glioblastoma-expressed RING finger protein) (RING finger protein 27) (RING-type E3 ubiquitin transferase TRIM8) (Tripartite motif-containing protein 8), TRIM8, GERP RNF27 |
| UniProt Protein Function: | TRIM8: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Belongs to the TRIM/RBCC family.Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 10q24.32Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; PML bodyMolecular Function: identical protein binding; protein binding; protein homodimerization activityBiological Process: activation of NF-kappaB transcription factor; innate immune response; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016] |
| UniProt Code: | Q9BZR9 |
| NCBI GenInfo Identifier: | 12382258 |
| NCBI Gene ID: | 81603 |
| NCBI Accession: | AAG53087.1 |
| UniProt Secondary Accession: | Q9BZR9,Q9C028, A6NI31 |
| UniProt Related Accession: | Q9BZR9 |
| Molecular Weight: | 61,489 Da |
| NCBI Full Name: | glioblastoma-expressed RING finger protein GERP |
| NCBI Synonym Full Names: | tripartite motif containing 8 |
| NCBI Official Symbol: | TRIM8 |
| NCBI Official Synonym Symbols: | GERP; RNF27 |
| NCBI Protein Information: | probable E3 ubiquitin-protein ligase TRIM8 |
| UniProt Protein Name: | Probable E3 ubiquitin-protein ligase TRIM8 |
| UniProt Synonym Protein Names: | Glioblastoma-expressed RING finger protein; RING finger protein 27; RING-type E3 ubiquitin transferase TRIM8Curated; Tripartite motif-containing protein 8 |
| Protein Family: | |
| UniProt Gene Name: | TRIM8 |
| UniProt Entry Name: |
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