| Kit Name: | TP (Thiamphenicol) ELISA Kit |
| Kit Size: | 96 Assays |
| Test Principle: | This kit uses Competitive-ELISA as the method. It can detect Thiamphenicol (TP) in samples, such as meat, eggs, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, TP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of TP. The concentration of TP in the samples can be calculated by comparing the OD of the samples to the standard curve. |
| Sensitivity: | 1 ppb (ng/mL) |
| Reaction Mode: | 25°C,30 min~30 min~15 min |
| Detection Limit: | Meat, Eggs ---1 ppb; Raw milk---1 ppb |
| Cross Reactivity: | Thiamphenicol---100%,Florfenicol---71%,Florfenicol Amine---7% |
| Sample Recovery Rate: | Muscle---90%±30%, Raw milk---100%±30% |
| Kits Components: | | Item | Specifications | | ELISA Microtiter Plate | 96 wells | | Standard Liquid | 1 mL each (0 ppb, 0.5ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb) | | HRP Conjugate | 7 mL | | Antibody Working Solution | 7 mL | | Substrate Reagent A | 6 mL | | Substrate Reagent B | 6 mL | | Stop Solution | 6 mL | | 20×Concentrated Sample Solution | 25 mL | | 20×Concentrated Wash Buffer | 25 mL | | Plate Sealer | 3 pieces | | Sealed Bag | 1 piece | | Manual | 1 copy | |
| Instruments: | Microplate reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01g). Nitrogen evaporators, Water bath. |
| Micropipette: | Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL). |
| Reagents: | Contact Technical Support |
| Storage And Expiry Date: | Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box. |
