Food Science

TMP (Trimethoprim) ELISA Kit (FSES0068)

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SKU:
FSES0068
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science

Description

Technical Manual

TMP (Trimethoprim) ELISA Kit

Kit Name: TMP (Trimethoprim) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Trimethoprim (TMP) in samples, such as raw milk, tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TMP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TMP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TMP. The concentration of TMP in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.15 ppb (ng/mL)
Reaction Mode: 25°C, 30min ˜15min
Detection Limit: Tissue---1pp; Raw milk ---4ppb
Cross Reactivity: TMP ---100%
Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1.5mL each (0ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb, 12.15ppb)
HRP Conjugate 7mL
Antibody Working Solution 7mL
10×Concentrated Sample Solution 10mL
Substrate Reagent A 7mL
Substrate Reagent B 7mL
Stop Solution 7mL
20×Concentrated Wash Buffer 25mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Methanol, NaHCO3.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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