TIFY10A Polyclonal Antibody (CAB21960)
- SKU:
- CAB21960
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant - Arabidopsis
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | TIFY10A Polyclonal Antibody |
| Product Code: | CAB21960 |
| Reactivity: | Arabidopsis thaliana |
| Applications: | Western blotting |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Reactivity: | Arabidopsis thaliana |
| Tested Applications: | WB, ELISA |
| Key Applications: | Western blotting |
| Recommended Dilution: | WB 1:500-1:1000 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Positive Samples: | Rosette leaf |
| Cellular Location: | nucleus, plasma membrane |
JAZ1 is a nuclear-localized protein involved in jasmonate signaling. JAZ1 transcript levels rise in response to a jasmonate stimulus. JAZ1 can interact with the COI1 F-box subunit of an SCF E3 ubiquitin ligase in a yeast-two-hybrid assay only in the presence of jasmonate-isoleucine (JA-ILE) or coronatine. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ1:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation. The Jas domain appears to be important for JAZ1-COI1 interactions in the presence of coronatine. Two positive residues (R205 and R206) in the Jas domain shown to be important for coronatine -dependent COI1 binding are not required for binding AtMYC2.
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 154-253 of arabidopsis thaliana TIFY10A (NP_564075.1). |
| Sequence: | SKGTA NSLAK NQTDI RSNIA TIANQ VPHPR KTTTQ EPIQS SPTPL TELPI ARRAS LHRFL EKRKD RVTSK APYQL CDPAK ASSNP QTTGN MSWLG LAAEI |
| Synonyms: | AtJAZ1, jasmonate-zim-domain protein 1, T29M8.5, T29M8_5, TIFY10A |
| Calculated MW: | 28kDa |
| Observed MW: | 32kDa |
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