Name: TIAM2 Antibody (PACO61418)
SKU: PACO61418
Availability: OutOfStock

Antibody Name:	TIAM2 Antibody (PACO61418)
Antibody SKU:	PACO61418
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human T-lymphoma invasion and metastasis-inducing protein 2 protein (1500-1627AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO61418 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with PACO61418 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.

Synonyms:

T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2, SIF and TIAM1-like exchange factor, TIAM2, KIAA2016, STEF

UniProt Protein Function:	Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (). Activates specifically RAC1, but not CDC42 and RHOA.
UniProt Protein Details:
NCBI Summary:	This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IVF5
NCBI GenInfo Identifier:	296453025
NCBI Gene ID:	26230
NCBI Accession:	Q8IVF5.4
UniProt Secondary Accession:	Q8IVF5,Q6NXN9, Q6ZUP9, Q9UFG6, Q9UKV9, Q9UKW0, B2RP56 C9JZV2
UniProt Related Accession:	Q8IVF5
Molecular Weight:	192,555 Da
NCBI Full Name:	T-lymphoma invasion and metastasis-inducing protein 2
NCBI Synonym Full Names:	T-cell lymphoma invasion and metastasis 2
NCBI Official Symbol:	TIAM2
NCBI Official Synonym Symbols:	STEF; TIAM-2
NCBI Protein Information:	T-lymphoma invasion and metastasis-inducing protein 2
UniProt Protein Name:	T-lymphoma invasion and metastasis-inducing protein 2
UniProt Synonym Protein Names:	SIF and TIAM1-like exchange factor
Protein Family:	T-lymphoma invasion and metastasis-inducing protein
UniProt Gene Name:	TIAM2
UniProt Entry Name:	TIAM2_HUMAN