TCEA3 Antibody (PACO59904)
- SKU:
- PACO59904
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | TCEA3 Antibody (PACO59904) |
| Antibody SKU: | PACO59904 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Transcription elongation factor A protein 3 protein (79-169AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59904 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO59904 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. |
| Synonyms: | Transcription elongation factor A protein 3 (Transcription elongation factor S-II protein 3) (Transcription elongation factor TFIIS.h), TCEA3, TFIISH |
| UniProt Protein Function: | TCEA3: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Belongs to the TFS-II family.Chromosomal Location of Human Ortholog: 1p36.12 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O75764 |
| NCBI GenInfo Identifier: | 28380172 |
| NCBI Gene ID: | 6920 |
| NCBI Accession: | O75764.2 |
| UniProt Secondary Accession: | O75764,Q5DR83, A8K2K7 |
| UniProt Related Accession: | O75764 |
| Molecular Weight: | 17,258 Da |
| NCBI Full Name: | Transcription elongation factor A protein 3 |
| NCBI Synonym Full Names: | transcription elongation factor A3 |
| NCBI Official Symbol: | TCEA3 |
| NCBI Official Synonym Symbols: | TFIIS; TFIIS.H |
| NCBI Protein Information: | transcription elongation factor A protein 3 |
| UniProt Protein Name: | Transcription elongation factor A protein 3 |
| UniProt Synonym Protein Names: | Transcription elongation factor S-II protein 3; Transcription elongation factor TFIIS.h |
| Protein Family: | Transcription elongation factor A protein |
| UniProt Gene Name: | TCEA3 |
| UniProt Entry Name: | TCEA3_HUMAN |
| Antibodies |
| TCEA3 Antibody (PACO12665) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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