TBARS Assay Kit (BA0060)

TBARS Assay Kit - Information

Assay Genie's TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 560nm/585nm) is directly proportional to TBARS concentration in the sample.

Applications

For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.

TBARS Assay Kit - Key Features

Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 , fluorometric assay 0.1 - 1.5 MDA.

TBARS Assay Kit - Data Sheet
Kit Includes	TBA Reagent: 25 mL Standard: 6 M MDA 10% Trichloroacetic acid (TCA): 25 mL
Kit Requires	Pipetting devices, centrifuge tubes, centrifuge, clear flat-bottom uncoated 96-well plates, optical density or fluorescence plate readers, sonicator, water-bath or heat block.
Method of Detection	OD535nm, or FL560/585nm
Detection Limit	0.1
Samples	Serum, plasma, urine, saliva etc
Species	All
Protocol Length	80 min
Size	100 tests
Storage	Store all components at -20 °C
Shelf Life	6 months

More Details

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery.